UHPLC/MS and NMR-Based Metabolomic Analysis of Dried Water Extract of Citrus-Type Crude Drugs

Citrus-type crude drugs (CCDs) are commonly used to formulate decoctions in Kampo formula (traditional Japanese medicine). Our previous study reported metabolomic analyses for differentiation of the methanol extracts using ultra-HPLC (UHPLC)/MS, and 13C- 1H-NMR. The present expanded scope its application by analyzing four CCD water (Kijitsu, Tohi, Chimpi, Kippi); these CCDs usually as decoction ingredients formula. A principal component analysis score plot processed UPLC/MS NMR data indicated that could be classified into three groups. loading plots showed naringin neohesperidin were distinguishing components. Three primary metabolites, α-glucose, β-glucose, sucrose identified compounds spectroscopy. During preparation dry extracts, some volatilized or decomposed. Consequently, fewer detected than our studies extract. However, results suggested combined NMR- LC/MS-based metabolomics can discriminate dried CCDs.